CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles

CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles

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Abstract The bean beetle (Callosobruchus maculatus) clearly exhibits population density-dependent polymorphism.Cytochrome P450 (CYP) is involved in many aspects of the physiological activities of insects.However, the role of CYP in population density-dependent jmannino.com polymorphisms remains unknown.

The terminal oocyte maturity rate of high-population-density individuals (H) was faster than that of low-population-density individuals (L).A total of 56 CYP-like genes were identified from transcriptomic and genomic data, including seven clan 2 CYP-like genes, seven mitochondrial CYP-like genes, 19 clan 3 CYP-like genes, and 23 clan 4 CYP-like genes.Gene duplication might occur in CYP9Z4-like, CYP345A1-like, CYP345A2-like, CYP349A1-like, CYP349A2-like, and CYP4BN4-like.

Thirteen and two CYP-like genes were up-regulated and down-regulated, respectively, in H.Among these CYP-like genes, CYP4BN4v7-like was the most abundant CYP.CYP4BN4v7-like was more highly expressed in the head than in the thorax and abdomen.

Its mRNA levels in the head, thorax, and abdomen were greater in H than in L.After RNA interference decreased its mRNA level, the terminal oocyte maturity rate decreased.Moreover, the expression level of insulin-like peptide 1 (ILP1), which plays a vital role in regulating terminal oocyte development, here decreased in the head.

In conclusion, CYP4BN4v7-like modulated the population density-dependent terminal oocyte maturity rate by regulating the expression of ILP1.